Isolation, Biochemical Characterization, PCR, Serotyping and Antibiogram of Salmonella from Chickens

Abstract

Salmonellosis is an infectious disease of Birds, Animals and Humans caused by organisms of the two species of Salmonella (Salmonella enterica, and S. bongori). Salmonellae produce Pullorum disease and Fowl Typhoid in poultry. In present study total of 260 tissue samples from 160 birds each Liver, Heart, Spleen and Gall Bladder, 40 eggs, 10 ovary samples, 25 faecal and feed samples from poultry farms in and around Bangalore were screened for Salmonella serovars prevalence. The isolation was carried out following OIE protocol of pre enrichment with BPW and enrichment with RVSM and TTBG. Selective plating was carried using BGA, XLD, HE and MCA. The BGA was found to be more efficient in isolation of salmonella when enriched with TTBG rather with RVSM than on XLD and HE. Better isolation rate with less number of false positive results were obtained on BGA with TTBG enrichment. A total of 21 isolates were obtained at the end, after series of Isolation, biochemical characterization, Serotyping and PCR. A prevalence of 8.05 per cent was recorded. Twenty one isolates were subjected for PCR with genus specific invA, serogroup specific sefA and serotype specific fliC primers. All isolates amplified with amplicon size for invA and sefA each but none from fliC and further PCR proved to be the rapid and sensitive test for identification of Salmonella.